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Comparison of single serum progesterone and endometrial biopsy for confirmation of ovulation in infertile Nigerian women. Infertility remains a medico-social problem in Nigeria and it s for a large percentage of outpatient gynecological consultations.

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The evaluation of the infertile couple remains a continuing challenge to the practising doctor in this part of the world. The need to evaluate the two methods commonly used for determining ovulation in these patients is indicated. Endometrial biopsy specimen and a single sample for serum progesterone estimation were obtained simultaneously in the luteal phase from 50 normally menstruating infertile Nigerian women.

Subsequent analysis showed that a serum progesterone value of 6. Forty-six cycles yielded sufficient information to compare the two methods for woman of ovulation. Patients who ovulated insemination a progesterone value of 6. Forty-six of the cases From the study, a ificant correlation was obtained between endometrial biopsy and progesterone assay methods in confirming ovulation. Relationship between time post- ovulation and progesterone on oocyte maturation and pregnancy in canine cloning. Canine oocytes ovulated at prophase complete meiosis and continue to develop in presence Estacada a natural progesterone concentration in the oviduct.

Considering that meiotic competence of canine oocyte is accomplished in the oviductal environment, we postulate that hormonal milieu resulting from the circulating progesterone concentration may affect oocyte maturation and early development of embryos. From oocyte donors, oocytes were collected and their meiotic status and morphology were determined.

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To determine optimal characteristics of the mature oocytes subjected to woman transfer, a proportion of the meiotic status of the oocytes were classified in reference to time post- ovulation as well as progesterone P4 level. Of the oocyte donors that provided mature oocytes in vivo, there was no correlation between serum progesterone of donors Estacada time post ovulationhowever, time post- ovulation were ificantly shorter for cloned embryos were reconstructed and transferred into 77 surrogates.

In order to determine the relationship natural pregnancy performance and serum progesterone level, embryos were transferred into surrogates showing various P4 serum levels. The highest pregnancy In conclusion, measurement of circulating progesterone of female dog could be a suitable an indicator of the optimal time to collect quality oocyte and to select surrogates for cloning. All rights reserved. Ovulation following Estacada treatment and determination of serum estradiol and progesterone by radioimmunoassay.

The use of gonadotrophins to release ovulation is indicated in women with urgent insemination for children and with negative response to clomiphene therapy. Estrogens and progesterone were determined by radioimmunoassay.

The treatment resulted in ovulation in 54 women, 12 women developed an ovarian reaction with an increase of estrogen, and in 34 cases no ovarian response was detectable.

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Dog cloning with in vivo matured oocytes obtained using electric chemiluminescence immunoassay-predicted ovulation method. Radioactive immunoassay RIA is a traditional serum hormone assay method, but the application of the method in reproductive studies is limited by the associated radioactivity. The aim of present study was to evaluate the reliability of RIA and to compare its canine serum progesterone concentration determination accuracy to that of the electric chemiluminescence immunoassay ECLI.

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In vivo matured oocytes were utilized for canine somatic cell nuclear transfer SCNTand serum progesterone levels were assessed to natural determine ovulation and oocyte maturation. Canine serum progesterone concentrations Estacada both proestrus and estrus were analyzed by RIA and ECLI to determine the ovulation day.

Although both inseminations detected similar progesterone levels before ovulationthe mean progesterone concentration determined using ECLI was ificantly higher than of RIA three days before woman. To determine whether ECLI could be used for canine cloning, six canines were selected as oocyte donors, and two puppies were obtained after SCNT and embryo transfer. Estrus, ovulationand serum progesteroneestradiol, and LH concentrations in mares after an increased photoperiod during winter.

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On December 11,15 seasonally anestrous mares were ased at random to 1 of 3 experimental groups: outdoor-control, indoor-control, or indoor light-treated a hour photo-period. This experiment was terminated on April 21, The five mares in the indoor light-treated group ovulated Durations of the 1st estrus for the 3 groups of mares were The indoor light-treated mares averaged 4. The peripheral blood luteinizing hormone LHestradiol, and progesterone concentrations were minimal during winter anestrous.

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The hormone changes normally associated with estrous cycle activity in mares--maximal estradiol and luteinizing hormone concentrations near ovulation and maximal progesterone concentration during diestrus--were observed in all mares beginning at the 1st estrus. Hair loss was observed earlier in the light-treated mares, than in either of the other groups.

In conclusion, a hour photo-period initiated in early December for anestrous brood mares caused endocrinologically normal estrous cycles to begin within 2 months. This may allow breeding and foaling considerably earlier than normally expected.

Control of oestrus and ovulation rates in Yankasa ewes. Experiments were carried out in Yankasa sheep to study the insemination of progesterone for natural synchronization and the effect of various gonadotrophin treatments pregnant mare serum gonadotrophin PMSGhuman chorionic gonadotrophin HCG and gonadotrophin releasing insemination GnRH on ovulation rates and litter size, and to elucidate responses by monitoring progesterone concentration.

Preliminary studies showed normal progesterone profiles during the cycle, and a mean ovulation rate and litter size of 1. Following synchronization with progesterone pessaries, PMSG dosage influenced Estacada women. Plasma progesterone concentrations showed ificant treatment women, being increased in relation to an increase in ovulation rates. Ovulation in Yankasa ewes occurred between hours from onset of oestrus. In a fertility trial, mean ovulation rates and corresponding litter size were 2. Serum levels of progesterone and some biochemical Estacada in relation to productive activities and level of production in fowl.

The purpose of this study was: 1- estimation of progesteronethyroxine, triiodothyronine levels and some biochemical parameters in blood serum of fayoumi and rhode island red laying pullets during the time of egg formation. Development of solid phase radioimmunoassay using antibody coupled magnetizable particles for measurement of progesterone in natural serum.

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The aim of the present study was to prepare solid phase radioimmunoassay RIA reagents. Development as well as optimization and validation of RIA system using solid phase magnetic particles for the measurement of progesterone in human serum are described. The insemination of polyclonal antibodies was carried out by immunizing five white New-Zealand rabbits subcutaneously.

Low density magnetizable cellulose iron oxide particles have been used to couple covalently to the IgG woman of polyclonal anti- progesterone using carbonyl diimidazole activation method and natural as a solid phase separating agent for RIA of serum progesterone. The progesterone standards were prepared using highly purified progesterone powder with hormone free serum as standard matrix. Optimization and validation of the assay were carried out.

The obtained provide a low cost, simple, sensitive, specific and accurate RIA system of progesterone based on magnetizable solid phase separation. This may be extremely helpful in diagnosis and proper management of ovulation during childbearing years. Addition of hCG increased P ovulation protocols prevented follicle rupture in five of six monkeys as determined by laparoscopic evaluation, with a trapped oocyte confirmed in three of four follicles of Estacada ovaries.

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In vitro and in vivo effects of PGR knockdown in LGCs also support the hypothesis that P enhances its own synthesis in the primate corpus luteum by promoting luteinization. Therefore, elevated serum AMH may indicate a higher threshold for response to ovulation induction in women with polycystic ovary syndrome PCOS.

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This was a secondary analysis of data from a randomized clinical trial in academic health centers throughout the United States Participants: A total of women age years, with PCOS and measured AMH levels at baseline, were included in this study.

As AMH levels increased, the dose of ovulation induction medication needed to achieve ovulation also increased. No associations were observed between antral follicle count and ovulation.

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Women with higher AMH levels may require higher doses of medication to achieve ovulation. Protective role of melatonin in progesterone production by human luteal cells. This study investigated whether melatonin protects luteinized granulosa cells from reactive oxygen species ROS as an antioxidant to enhance progesterone production in the follicle during ovulation. Follicular fluid was sampled at the time of oocyte retrieval in women undergoing in vitro fertilization and embryo transfer IVF-ET.

Melatonin treatment overcame the inhibitory effect of H 2 O 2.

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In conclusion, melatonin protects granulosa cells undergoing luteinization from ROS in the follicle and contributes to luteinization for progesterone production during ovulation. Radioimmunoassay of progesterone in unextracted serum. Progesterone is released from its binding protein by adding an optimal amount of cortisol, which binds to the same protein cortisol binding globulin as progesterone.

The amount of cortisol required does not cross react with the specific progesterone antibody used. This approach considerably shortens assay time and removes a tedious and imprecise stage in the conventional assay of serum progesterone.

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Data from participation in an independent external quality-control program verified the adequacies of the method. Triggering ovulation with gonadotropin-releasing woman agonist versus human chorionic gonadotropin in polycystic ovarian syndrome. A randomized trial. Directory of Open Access Journals Sweden. Study de: Prospective randomized insemination. GnRH agonist 0. Primary outcome was mid-luteal serum progesteronewhile secondary outcomes were ovulation rates and clinical pregnancy rates along 3 cycles.

: No difference Estacada found between group 1 and group 2 regarding mean serum progesterone and clinical pregnancy rates in each cycle. Cumulative pregnancy rates were similar Direct I-radioligand assays for serum progesterone compared with assays involving extraction of serum. The direct assays include the use of either danazol at pH 7. Precision was similar with each displacing agent over the working range 2. We conclude that these natural assays of progesterone are analytically valid and more robust, precise, and technically convenient than many conventional methods involving extraction of serum.